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Preferential activation of Notch2 or Notch1 by the stimulation with Dll1-Fc or Jagged1-Fc. Osteoclasts were differentiated from mouse BM as described in Figure 2c. ICDs of Notch1 and Notch2 on day 1 were analyzed by Western immunoblotting. TBP was used as a loading control. N1, Notch1; N2, Notch2. Similar results were obtained in three independent experiments.
