Table 1.
Distribution of FRβ in normal rat tissues analyzed using flow cytometry or immunohistochemistry
| Flow Cytometric Analysis | FRβ | CD11 b/c |
|---|---|---|
| TGC-elicited Peritoneal mϕ | 87 ± 3.1% | 95 ± 2.5% |
| Immunohistochemical Analysis | FRβ | CD68 |
| Spleen | ||
| White pulp | - | ++ |
| Red pulp mϕ | + | +++ |
| Lymph node | ||
| Cortex, tingible body mϕ | - | ++ |
| Paracortical mϕ | - | + |
| Medullary mϕ | - | + |
| Subcapsular sinus mϕ | - | + |
| Brain mϕ | - | + |
| Liver mϕ (Kupffer cells) | + | ++ |
| Lung | ||
| Perivascular/peribronchial mϕ | - | + |
| Alveolar mϕ | - | + |
| Kidney | ||
| Mesangial mϕ | - | + |
| Heart | ||
| Myocardial mϕ | - | + |
| Endocardial mϕ | - | + |
| Colon | ||
| Mucosa | ||
| Epithelial mϕ | - | + |
| Lamina propria mϕ | + | + |
| Muscularis mucosa mϕ | - | + |
| Submucosal mϕ | - | + |
| Muscularis externa mϕ | - | + |
Frequency of cell staining (-, <100/10 fields), (+, 100-200/10 fields), and (++, 200-300/10 fields), (+++, >300/10 fields). Data was obtained from three normal rat tissues with similar results. FRβ, folate receptor β; TGC, thioglycollate.