Table 1.
Sensitivity and specificity of our new IP-qPCR method.
| Autoantibody specificitya | n | Anti-Th/To by IP-qPCR |
Anti-U3RNP by IP-qPCR |
|---|---|---|---|
| Scleroderma | |||
| Th/To | 22 | 100% | 4.5% (1/22) |
| U3RNP | 12 | 0% | 100% |
| Topo I | 20 | 0% | 0% |
| RNAPIII | 18 | 0% | 0% |
| ACA | 15 | 0% | 0% |
| PM-Scl | 5 | 0% | 0% |
| Others | |||
| TMG | 3 | 0% | 100% |
| La | 12 | 0% | 42% (5/12) |
| NHS | 15 | 0% | 0% |
Results show the high sensitivity and specificity of the new assay, for the detection of anti-Th/To and -U3RNP antibodies, compared with silver staining as gold standard. aAntibody specificity validated by IP, urea-PAGE, and silver staining. ACA, anti-centromere antibodies; IP, immunoprecipitation; NHS, normal human serum; RNAPIII, RNA polymerase III; TMG, trimethylguanosine; topo I, topoisomerase I.