Table 1.
QPCR of chondrogenic gene expression in cartilage/subchondral bone
| acan | P | Fold4 | col2a | P | Fold4 | col102a | P | Fold4 | |
|---|---|---|---|---|---|---|---|---|---|
| ΔCT1 | ΔCT1 | ΔCT1 | |||||||
| Naive | 3.84 (0.30) | 1.00 | 4.29 (0.25) | 1.00 | 6.33 (0.08) | 1.00 | |||
| Acute | 2.58 (0.04) | .00012 | 2.39 | 1.76 (0.38) | .00012 | 5.77 | 6.74 (0.39) | NS2 | 0.75 |
| TTR | 3.59 (0.34) | NS2 | 1.90 | 2.82 (0.60) | .00142 | 2.77 | 6.59 (0.42) | NS2 | 0.83 |
| TTR+HA | 2.13 (0.24) | .00013 | 3.27 | 0.86 (0.19) | .00033 | 10.7 | 4.82 (0.30) | .00013 | 2.85 |
| TTR+SA | 3.36 (0.03) | NS3 | 1.40 | 2.32 (0. 4) | NS3 | 3.92 | 6.16 (0.43) | NS3 | 1.12 |
1relative to GAPDH, mean (SD) ΔCT of 8 pools with 2 mice per pool for Naïve and 12 pools with 2 mice per pool for all 4 treatment groups.
P-values: 2Acute or TTR vs Naive; 3TTR+HA or TTR+SA vs TTR;
4Fold Changes are relative to Naive
NS, not significant