Table 2.
QPCR of fibrogenic gene expression in cartilage/subchondral bone
| col1a | P | Fold4 | col3a | P | Fold4 | col5a | P | Fold4 | |
|---|---|---|---|---|---|---|---|---|---|
| ΔCT1 | ΔCT1 | ΔCT1 | |||||||
| Naive | -0.84 (0.23) | 1.00 | 6.67 (0.17) | 1.00 | 9.98 (0.36) | 1.00 | |||
| Acute | -6.03 (0.29) | < .00012 | 36.5 | 5.10 (0.71) | .0032 | 2.97 | 8.50 (0.41) | .0122 | 2.80 |
| TTR | -6.02 (1.03) | < .00012 | 36.2 | 2.95 (0.44) | .00012 | 13.2 | 5.82 (1.02) | .0012 | 17.9 |
| TTR+HA | -2.44 (0.52) | .00023 | 3.03 | 5.08 (0.59) | .00123 | 3.03 | 7.20 (0.44) | .0293 | 6.87 |
| TTR+SA | -7.25 (1.84) | NS3 | 84.4 | 3.15(1.09) | NS3 | 11.5 | 4.87 (0.65) | NS3 | 34.5 |
1relative to GAPDH, mean (SD) ΔCT of 8 pools with 2 mice per pool for Naïve and 12 pools with 2 mice per pool for all 4 treatment groups.
P-values: 2Acute or TTR vs Naive; 3TTR+HA or TTR+SA vs TTR;
4Fold Changes are relative to Naive
NS, not significant