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. 2012 Sep;86(18):9919–9928. doi: 10.1128/JVI.00388-12

Fig 4.

Fig 4

Inhibition of HLA-DR expression on hepatocytes by HCV proteins. (A) HCV core- or NS5A-transfected Huh7 cells were stimulated with 500 U/ml of IFN-γ for 48 h and analyzed for HLA-DR cell surface expression after immunostaining with FITC-conjugated specific antibody. Cells stained with the FITC-mouse IgG2a(κ) isotype control are shown as the negative control. (B) Real-time PCR analysis for HLA-DR mRNA status in HCV gene-transfected IHH treated with IFN-γ. (C) HLA-DR mRNA status in chronically HCV-infected human liver biopsy specimens. The mRNA level was normalized with 18S RNA. The results are shown as means and standard deviations from triplicate assays. The P value was determined by using a two-tailed t test: *, P < 0.05; **, P < 0.01 compared to vector control or normal liver biopsy samples.