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. 2012 Sep;86(18):10047–10058. doi: 10.1128/JVI.01140-12

Fig 4.

Fig 4

Localization of E2 to pericentromeric foci in mitotic and interphase cells. E2 protein expression was induced in CV-1 cell lines expressing the 240-255-CTD E2 protein for 3 h. (A) E2 proteins were detected in mitotic cells using anti-FLAG antibody (green), centromeres were detected with anti-ACA antibody (blue), rDNA loci were detected with anti-UBF (red), and cellular DNA was stained with DAPI (gray). (B) (Left) Wild-type 240-255-CTD E2 protein was detected using anti-FLAG antibody (green), and the rDNA loci were detected with anti-UBF (red); (center) wild-type 240-255-CTD E2 protein was detected using anti-FLAG antibody (green), and the centromeres were detected with anti-ACA antibody (red); (right) the S253A 240-255-CTD E2 was detected using anti-FLAG antibody (green), and the centromeres were detected with anti-ACA antibody (red). mt, S253A mutation in E2.