Fig 9.

Residues arginine 250 and serine 253 are critical for the association of full-length HPV8 E2 with mitotic chromosomes. (A) CV-1 cell lines carrying control vector pMEP4 or plasmids expressing wild-type HPV8 E2 (WT), R250-mutated E2 (R250A), or S253-mutated E2 (S253A) were visualized by indirect immunofluorescence to determine the location of E2 proteins on the mitotic chromosomes. E2 proteins were detected using anti-FLAG antibody (green), and cellular DNA was stained with DAPI (blue). (B) The percentage of mitotic cells containing E2 bound or excluded from the chromosomes is shown for each protein. Results are from three independent experiments, and values were adjusted for the percentage of interphase cells expressing E2. The phenotype of the double mutation RSAA (R250A and S253A) is also shown.