Fig 6.

Effect of VPg point mutations on viral replication. Immunofluorescence analysis of astrovirus replication on BHK-21 cells transfected with capped transcripts derived from the wild-type (Wt) plasmid (pAVIC) or the corresponding plasmids containing the engineered mutations in the VPg coding region (panel 1: Y693A; panel 2: Y720A; panel 3: Y723A; panel 4: Y728A; panel 5: Y729A; panel 6: Y747A; panel 7: L701I; panel 8: Y693S; panel 9: Wt). Detection of astroviral structural proteins on transfected BHK-21 cells was performed at 24 h posttransfection using the 8E7 MAb. Magnification, ×200.