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pERK is specifically incorporated into the tegument of RRV virions. Gradient-purified RRV was either left untreated (lane 1) or treated with proteinase K (150 ng/ml; lane 2) or Triton X-100 (2%; lane 3) and analyzed by immunoblotting alongside capsids purified from isolated nuclei (Nuc cap; lane 4), probing for the presence or absence of pERK (bottom panel) and MCP (top panel), the latter to approximate the relative particle number in each sample.
