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An MVA virus lacking C11R (MVAΔC11R) reduces ERK2 activation. 293T cellular monolayers were either mock infected or infected with either MVA or MVAΔC11R (MOI, 10). At either 1, 2, 3, 4, 5, or 6 h p.i., cells were lysed, and 20 μg of cytoplasmic extracts from each sample was analyzed by immunoblotting, using antibodies recognizing the phosphorylated form of ERK2 (p-ERK1/2). The blot was reprobed with antibodies recognizing ERK1/2.
