Fig 5.

Interferon induction by Ad35 and Ad26 in the presence of anti-CAR or anti-CD46 blocking MAbs. Normal human PBMC (n = 4 to 6 per group) were preincubated with 10 μg/ml anti-CAR (RmcB) or anti-CD46 (13/42 or M177) for 1 h and then stimulated with 10³ vp/cell of Ad35 or Ad26 vector. IFN-α2 and IFN-γ levels were measured 24 h following stimulation by Luminex assays. LPS (1 ng/ml) was included as the positive control. Data are mean responses and the SEM. Bars indicate P values of <0.05 (dotted lines), P values of <0.01 (dashed lines), or P values of < 0.001 (solid lines) using Kruskal-Wallis tests with Dunn's correction for multiple comparisons. Unstim., unstimulated.