Fig 1.

HCV infection enhances miR-130a expression. (A) Immortalized human hepatocytes (IHH) were infected with HCV genotype 1a for 72 h. RNA was isolated, and miR-130a was measured by real-time PCR. U6 RNA was used as an internal control. The results from three independent experiments are presented. (B) Total RNA was extracted from HCV-infected and control liver biopsy specimens (not infected with HCV or HBV). miR-130a expression was analyzed by real-time PCR, and U6 RNA was used as an internal control. A comparison of miR-130a levels in control (n = 8) and HCV-infected (n = 9) liver tissues is shown. (C) Results from individual specimens of panel B are shown. HCV loads from each specimen are mentioned on the top of each bar. (D) IFITM1 expression from control (n = 6) and HCV-infected (n = 6) liver biopsy specimens after normalization with internal control 18S RNA is shown.