Fig 2.

Knockdown of miR-130a enhances IFITM1 expression in hepatocytes. (A) Huh7 cells were treated with a control or anti-miR-130a. Cell lysates were prepared after 48 h of transfection, and IFITM1 expression was analyzed by a Western blot using a specific antibody. The blots were reprobed with an antibody to actin for a comparison of protein loads. The fold change of IFITM1 after normalization with actin is shown and compared with the control-treated sample. (B) IFITM1 mRNA was measured from control and miR-130a knockdown cells as described in Fig. 1. The results are the means of three independent experiments. (C and D) Huh7.5 cells were infected with HCV and were treated with the control or anti-miR-130a. HCV and IFITM1 mRNA levels were measured by real-time PCR and presented after normalization with GAPDH. The results shown are from two independent experiments with three replicates. (E) HCV-infected cells transfected with the control or anti-miR-130a. Cells were examined for miR-130a expression by qRT-PCR. Results after normalization with U6 as an internal control are presented.