Table 1.
Infectious titers of the recombinant PICV Z mutants generated by reverse genetics systema
| WT or mutant | Z mutation | Infectious titers (PFU/ml) |
|||
|---|---|---|---|---|---|
| 48 hpt | 72 hpt | 96 hpt | 120 hpt | ||
| WT | WT | 2,600 | 4.8E + 04 | 7.8E + 05 | 1.2E + 07 |
| M32 | G2A | 0 | 0 | 0 | 0 |
| M24 | C38A/C41A | 0 | 0 | 0 | 0 |
| M26 | C51A/H54A/C57A/C60A | 0 | 0 | 0 | 0 |
| M28 | C71A | 0 | 0 | 0 | 0 |
| M34 | P88A/S89A | 0 | 140 | 1,600 | 4,400 |
| M36 | P90A/P91A | 0 | 240 | 1,800 | 4,000 |
| M38 | E94A/P95A | 0 | 0 | 160 | 720 |
| M30 | LPTK(78–81)/AAAA | 0 | 0 | 0 | 0 |
| M914 | L78A | 0 | 0 | 0 | 0 |
| M916 | P79A | 0 | 0 | 0 | 0 |
| M918 | T80A | 12 | 680 | 3,000 | 2.4E + 04 |
| M40 | K81A | 120 | 2,600 | 4.2E + 04 | 3.6E + 05 |
a
Recombinant PICV strains were generated by the reversegenetics systems (14), in which a plasmid encoding thefull-length L segment of either the WT or the respective mutant Z gene, alongwith a plasmid encoding the S segment, were transfected into BSRT7-5cells. Supernatants collected at 48, 72, 96, and 120 h posttransfection (hpt)were subjected to plaque assay to determine the titer of infectious virus. Theresults shown are representative of three independent experiments.