Fig 1.

Representative flow cytometry data. (A) Gating scheme for CD4 T-cell intracellular cytokine cytometry. FSC-A, forward scatter A; SS, side scatter. Black polygons indicate gated cell subsets. (B) Responses of PBMC from an HSV-2-seropositive individual. At left, cells were stimulated with UV-HSV-2 and stained with isotype control MAb. The remaining panels show cells treated with the indicated stimuli and stained with the anticytokine MAb. The numbers are the percentages of gated CD4 T cells in the respective quadrants. (C) pDC enumeration analysis for a representative subject. Black polygons in the left 2 dotplots show cell gating. The right 3 dotplots show the percentages of gated cells positive for the indicated markers enclosed in the small polygons. (D) Gating scheme for DC ICC analyses. The third dotplot shows boxed CD123^high CD11c^neg cells. The fourth dotplot shows the final pDC gate of HLA-DR^pos lineage^neg cells. (E) Responses of gated pDC to media, live HSV-2 at MOI 100, and TLR agonists or controls. Numbers in quadrants are percentages of gated cells expressing the indicated patterns of cytokines.