Fig 4.

Intracellular cytokine cytometry results for circulating CD4 T-cell expression of IFN-γ for specimens collected during and after symptomatic genital HSV recurrences in five subjects. Peripheral blood mononuclear cells (PBMC) were collected between 3 and 13 times per participant over a course of 5 to 122 days. Results are expressed as percentage of circulating live CD3⁺ CD8⁻ CD4⁺ T cells expressing IFN-γ. PBMC were collected and frozen, and then all samples for each participant were run on the same day. Red data points are times at which HSV-2 was detected by PCR from genital specimens.