Fig 5.

Peptide competition neutralization assays. Percent neutralization versus MAb concentration or plasma dilution is shown. (A) Serially diluted MAb 2F5 was preincubated with 2F5 peptides prior to addition of F535 M^sens in the neutralization assay. (B) S208 maternal plasma was preincubated with either V4 or HR2 peptides before addition of S208 M^sens1 or M^sens2, respectively. (C) F535 maternal plasma was preincubated with V1, V2, V3, or V1/V2 peptides before addition of F535 M^sens. Contribution of peptides to maternal plasma or 2F5 neutralizing activity was calculated as percent reduction in AUC relative to 2F5 or plasma neutralization in the absence of peptides. Values for average percent reduction in AUC from at least 2 independent experiments are shown in parentheses in the symbol keys.