Fig 8.

Protein competition neutralization experiments. Serially diluted MAb VRC01 (A) or maternal plasma (B and C) was preincubated with RSC3 or RSC3Δ371I before use in neutralization assays against S208 M^sens1, S208 M^sens1 and S208 M^sens2, or F535 M^sens. The percent neutralization versus MAb concentration or plasma dilution is shown in each panel. The contribution of antibodies against the CD4 binding site to overall neutralizing activity of VRC01 or maternal plasma was calculated as percent reduction of AUC relative to neutralization by VRC01 or plasma in the absence of protein. Values for average percent reduction in AUC from at least 2 independent experiments are shown in parentheses in the symbol keys.