Fig 3.

Effects of a PHx on HBV, mediated by IFN-α/β. (A) Induction of 2′,5′-OAS expression by a PHx. Total liver RNA was extracted from mice 1 day (D1) after the PHx for the analysis of the 2′,5′-OAS mRNA by semiquantitative reverse transcription-PCR (RT-PCR). The resected liver was used as the day 0 (D0) control. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) mRNA was also analyzed to serve as an internal control of RT-PCR. (B) Effects of anti-IFN-α/β antibodies and a PHx on HBV. Mice were injected with 200 μg of the control IgG or the anti-IFN-α/β antibodies as indicated, followed by a PHx. Mice were sacrificed 3 days after the PHx for the isolation of the liver for HBV RI DNA, RNA, and core protein analyses. Note that the order of samples in lanes 9 to 12 is slightly different from that in lanes 1 to 8.