Fig 4.

Mapping UL99 interaction domains. (A) Yeast cells were cotransformed with bait plasmid expressing wild-type UL99 or the indicated UL99 mutant and prey plasmid expressing UL94. Interactions were assessed by growth of cotransformed cells on medium lacking histidine. (B) 293T cells were cotransfected with plasmid DNA expressing HAUL94 and the indicated FLAGUL99. Total protein was harvested at 48 h posttransfection, and immunoprecipitation was performed with anti-FLAG antibody. Immune complexes were separated by SDS-PAGE, and Western blotting was performed with anti-HA antibody.