Table 2.
Summary of the growth phenotypes of 16 alanine-scanning mutants of 2Ca
| Group (no. of mutants with indicated phenotype) | Alanine-scanning mutant (mutations) | Time of full CPE at indicated temp (°C)b |
Translation and polyprotein processing | P2/2BC/2C migration on SDS-PAGE gel | Replication | Reversion(s)/suppressor mutation(s) | Defect(s) | ||
|---|---|---|---|---|---|---|---|---|---|
| 33 | 37 | 39.5 | |||||||
| I (3) Wt like | 2 (K33A/E34A/K35A) | Tf | Tf | Tf | + | + | + | − | None |
| 4 (K49A/R51A) | Tf | Tf | Tf | + | Shift | + | − | None | |
| 10 (R256A/D257A) | Tf | Tf | Tf | + | + | + | − | None | |
| II (1) ts | 7 (E148A/R149A/E150A) | Tf | None or passage 1 | None | + | Shift | ts | 2C | ts, replication |
| III (10) lethal | 3 (R41A/D42A/K43A) | None | None | None | + | + | − | − | Replication |
| 5 (K88A/R89A) | None | None | None | + | + | − | − | Replication | |
| 6 (E97A/K99A/R100A) | None | None | None | + | Shift | − | − | Replication | |
| 8 (R240A/R241A) | None | None | None | + | Shift | − | − | Replication | |
| 9 (D245A/D247A) | None | None | None | + | + | − | − | Replication | |
| 12 (D294A/K295A) | None | None | None | + | + | − | − | Replication | |
| 13 (R298A/R300A) | None | None | None | + | Shift | − | − | Replication | |
| 14 (E313A/R314A/R316A/R317A) | None | None | None | + | Shift | − | − | Replication | |
| 15 (D183A/D186A/K188A) | None | None | None | + | Shift | − | − | Replication | |
| 16 (E207A/E208A/K209A) | None | None | None | + | Shift | − | − | Replication | |
| IV (2) q.i. | 1 (K6A/K7A) | Passage 2 | Passage 1 | Passage 1 | + | + | q.i. | 2C | Replication |
| 11 (K279A/R280A) | Passage 1 | Passage 1 | None or passage 1 | + | Shift | q.i. | 2C, VP3, VP1 | Replication and Encapsidation | |
a
ts, temperature sensitive; q.i., quasi-infectious.
b
Tf, full CPE at transfection; None, no CPE; None or passage 1, no CPE or full CPE at passage 1.