Fig 6.

Alternative and classical pathway DAF activity associated with MuV and VSV particles. Purified MuV (A and C) or VSV (B and D) that was derived from the indicated cells was assayed for decay-accelerating activity against the alternative pathway (AP) (A and B) or classical pathway (CP) (C and D) C3 convertase as detailed in Materials and Methods. C3-mediated hemolytic activity is expressed as a percentage of the lysed control sample set at 100%. Data are from triplicate samples, and error bars represent standard deviations. (A and B) #, P < 0.001 when comparing results for 0.25 to 1 μg of MuV-CD55 and MuV-CD46/55 with those for 0.25 to 10 μg from control virus derived from CHO-K1 cells or 0.25 to 1 μg of VSV-CD55 and VSV-CD46/55 with those for 1 to 5 μg from control virus derived from CHO-K1 cells; (C and D) #, P < 0.001 when comparing results for 0.5 to 2.5 μg VSV-CD55 with those for 1 to 10 μg control virus and comparing results for 2.5 to 10 μg MuV-CD55 and MuV-CD46/55 with those for the corresponding amount (μg) of control virus; *, P < 0.01 when comparing results for 0.25 to 10 μg VSV-CD46/55 with those for 1 to 10 μg control virus derived from CHO-K1 cells.