Table 1.
Virusa | Levelb (ng) |
|
---|---|---|
CD55 | CD46 | |
MuV CHO-CD46 | 20 | |
MuV CHO-CD55 | 25 | |
MuV CHO-CD46/55 | 10 | 10 |
VSV CHO-CD46 | 25 | |
VSV CHO-CD55 | 50 | |
VSV CHO-CD46/55 | 25 | 25 |
Viruses were grown in CHO cells expressing CD46, CD55, or CD46/55 and purified by gradient centrifugation.
Levels of CD55 and CD46 were determined by Western blotting and compared to standards of purified protein.