Fig 8.

Purification, hemagglutination (HA), and binding to saliva samples of the triple-layered particles (TLPs) of a P [9] rotavirus (Arg 720 strain). (A) CsCl gradient purification with indication of the TLP band. (B) The TLP band (density, 1.36 g/cm³ in CsCl) was confirmed by electron microscopy (EM) examination. (C) HA of the TLP virions. Twofold serial dilutions of TLPs were used in the HA assay, with PBS buffer without TLPs as the negative control (NC). The TLPs were found to specifically agglutinate the type A RBCs. (D) Specific binding of the TLPs to the type A saliva samples. The x axes show a panel of A-positive and -negative saliva samples, and the y axes show the optical density (OD) value of the binding signals. Error bars show the standard deviations from two experiments.