Alignment of zoonotic HEV PPRs. Sequences were aligned in Clustal X2 and adjusted manually. Purines, black background with white text; pyrimidines, gray background with black text; dashes represent gaps. Sequence IDs are prefixed with their genotype: 3, genotype 3; 4, genotype 4; 5, Japanese wild boar sequences; R, Chinese rabbit sequences. (A) Sequences aligned from nucleotide position 2121. (B) Sequences aligned from the carboxyl-terminal conserved sequence. A ruled guide is attached to each alignment.