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. 2012 Sep 10;134(37):15213–15216. doi: 10.1021/ja306061x

Figure 3.

Figure 3

Comparison of the kinetics of cluster transfer from A. vinelandii [Fe2S2]-IscU to apo-Grx5 in the presence and in the absence of HscA/HscB/ATP. All conditions are the same as described in Figures 1 and 2. The data in the presence of HscA/HscB/ATP (■) were obtained by continuously monitoring the CD intensity at 460 nm after initiation of the reaction with Mg-ATP, and the solid line is a best-fit simulation to second-order kinetics with a rate constant of 20 000 M–1 min–1. The data in the absence of HscA/HscB/ATP (•) were obtained by monitoring the difference in the CD intensity at 457 and 408 nm, and the solid line is a best-fit simulation to second-order kinetics with a rate constant of 30 M–1 min–1.