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. 2012 Sep 19;103(6):1370–1378. doi: 10.1016/j.bpj.2012.08.032

Figure 1.

Figure 1

Structural models of SERCA (left, crystal structure 1IWO (76)) and monomeric PLB (right, hybrid NMR structure 2KB7 (77)). All experiments in this study were conducted with the monomeric form of PLB. The amino-acid TOAC (bottom right), containing a nitroxide spin label rigidly coupled to the α-carbon, is incorporated at position 36 (shaded star) on PLB; PLB residues 41 and 46 (shaded) are mutated to Phe and Ala. For unlabeled PLB, position 36 is Ala. For spin-labeled SERCA, MSL (top right) is attached at Cys344/Cys364 (solid star) (78).