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Dependence of conventional EPR spectra of 36-TOAC-PLB on lipid/protein ratio. (a) Spectra of 36-TOAC-PLB reconstituted at 200–1000 L/P. (b) Outer splitting (2T||′, triangles) and low-field outer linewidth (ΔL′, squares) as a function of L/P. The vertical scales were set so that a change in rotational motion would yield changes in ΔL′ and 2T||′ of similar magnitude (but opposite sign) (49,50). STEPR spectra are given in Fig. S3.
