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Effects of Ser16 phosphorylation and SERCA on EPR of 36-TOAC-PLB. (a) Conventional (V1) EPR spectra and (b) STEPR (V2′) spectra of 36-TOAC-PLB as affected by phosphorylation (pPLB) and/or SERCA (0.5 PLB/SERCA). (c) Outer splitting 2T||′ measured from conventional EPR spectra in panel a. (d) Effective rotational correlation times from STEPR spectra in panel b. Typical normalized spectra are shown in panels a and b. (Bar graphs in panels c and d show mean ± SE for n = 3–5 trials.)
