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STEPR (V2′) spectra of MSL-SERCA. Experimental conditions are as in Fig. 6, using MSL-SERCA and unlabeled PLB. Rotational correlation times did not vary significantly among these samples and were consistent with the data in Fig. 5 at 700 lipids/SERCA (13 ± 4 μs). Conventional EPR spectra also showed no significant changes (see Fig. S5).
