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Histograms of the FRET efficiency created from nine sets of smFRET time series. (Green histogram) Constructed from the FRET trajectories with a 5-ms bin, calculated directly from the fluorescence signals, forms a single broad peak. Individual states are not distinguishable. (Blue histogram) Constructed from the VB-HMM-TS-FRET results, in which three distinct distributions are clearly shown. (Color online.)
