FIGURE 4.
Dependence of the discrimination between bound and unbound sites on the location of the nucleation region within the seed match. (A) Numbering of the positions in the binding site. It is based on the corresponding numbering of positions in the miRNA. (B–G) Discrimination obtained when the nucleation region within the binding site is restricted to the positions indicated on the x-axis. For each length n, all possible locations were explored, always using the Pcutoff value indicated in Table 1. Statistical significance was evaluated with the unpaired one-sided t-test.