FIGURE 2.

The MRB8170 and MRB4160 proteins bind RNA. Proteins incubated with the first 102 nt of pre-edited cyB sequence body-labeled with [α-³²P]UTP were UV-cross-linked, treated with RNase A, and separated on a 10% SDS-acrylamide gel. (A) Autoradiograph of recombinant proteins binding radiolabeled single-stranded RNA. (B) Coomassie-stained 10% SDS-acrylamide gel. The band indicated as “b” in lane 1 is BSA, a component of the binding buffer. (*) Bands corresponding to recombinant proteins indicated at the top of the lanes. (#) The 25-kDa band in lane 8 that stains the free GST-tag. Protein ladder sizes are indicated on the right of both panels.