FIGURE 3.

MRB8170 appears to preferentially bind mRNA in vitro. Binding curves of MRB8170 binding to (A) pre-edited RPS12 mRNA, (B) edited RPS12 mRNA, and (C) gA6[14] gRNA, which possesses a U₁₇ 3′-oligomer tail. The range of protein concentrations used is given on the x-axis in nanomolars and the fraction of bound RNA on the y-axis. The apparent K_d determined from the fitted curves is given along with the B_max of MRB8170 RNA binding and coefficient of determination (R²) of each curve. Whiskers at each data point represent standard deviation over two separate experiments done in triplicate.