FIGURE 7.

Simultaneous down-regulation of MRB8170 and MRB4160 leads to disruption of macromolecular complexes containing some MRB1 subunits. Mitochondrial vesicles from the double and single MRB8170/4160 knockdown cell lines were isolated following RNAi induction by tetracycline (Tet+). Parallel samples grown for this time in the absence of the antibiotic were also analyzed (Tet−). The mitochondrial proteins from MRB8160/MRB4160 dKD (A), MRB8170 sKD (B), and MRB4160 sKD (C) were resolved by glycerol gradient sedimentation and analyzed by Western blot as described in Figure 4. The column marked as “mito” contains total mitochondrial lysate. The identity of the antibody used is indicated at the right.