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. 2012 Oct;18(10):1875–1885. doi: 10.1261/rna.034538.112

FIGURE 2.

FIGURE 2.

The preE of let-7 is sufficient to direct both Lin28 binding and uridylation of pre-let-7. (A) Diagram of synthetic RNAs used for in vitro uridylation assays. (Pre-let-7g) Endogenous precursor let-7g miRNA sequence; (Pre-miR-21) endogenous precursor miR-21 sequence; (Pre-7S21L) synthetic RNA consisting of the miR-21 preE and let-7g stem; (Pre-21S7L) synthetic RNA consisting of the let-7g preE and miR-21 stem sequences. (B, left) Uridylation assay as in Figure 1 using WT Flag IP-mZcchc11, with or without Flag IP-mLin28, and the indicated precursor miRNAs. (Right) 5′ end-labeled RNAs showing equal amounts. (C) (Top) Diagram of synthetic RNAs used for in vitro uridylation assays. (Bottom left) Uridylation assays and (bottom right) 5′ end-labeled RNAs showing equal amounts as in B.