Figure 3.

Effect of dehydroascorbic acid on the cell viability. Confluent monolayers of HEp-2 cells were incubated at 37°C in MEM containing 0.1% BSA and varying concentrations of dehydroascorbic acid. After incubation for 24 h, the treated cells were trypsinized to obtain a single-cell suspension, and the amounts of living and dead cells in each culture were determined by a dye-exclusion test with trypan blue.