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. 2012 Sep 11;2012:854502. doi: 10.1155/2012/854502

Figure 4.

Figure 4

Overexpression of CG6783 or CG13890 activates dFOXO. (a) Overexpression of CG6783 or CG13890 induced the translocation of dFOXO to the nucleus. The fat bodies of the third instar larvae expressing CG6783 or CG13890 under da-Gal4 were stained with anti-dFOXO (green) and DAPI (blue). White arrows indicate dFOXO-negative cells and red arrows indicate dFOXO-positive cells. Original magnification is 200x. (b) Overexpression of CG6783 or CG13890 increased the expression of dFOXO target genes. The mRNA of l(2)efl and 4E-BP was analyzed in the whole body RNA extract from da > EP (black or dark gray bars) and da/+ (gray bars) flies. Significance was determined via a t-test (*P < 0.01, **P < 0.001).