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. Author manuscript; available in PMC: 2012 Sep 19.
Published in final edited form as: Gastroenterology. 2007 Mar 24;132(5):1834–1851. doi: 10.1053/j.gastro.2007.03.038

Figure 3.

Figure 3

Activation of prosurvival signaling pathways by LPA and OTP in IEC-6 cells. IEC-6 cells were pretreated with PTX (50 ng/mL) overnight, PD98059 (20 µmol/L) for 1 hour, or LY294002 (10 µmol/L) for 30 minutes, followed by the addition of 10 µmol/L OTP or LPA. Activation of (A) ERK1/2, (B) P38 mitogen-activated protein kinase, and (C) PKB/ AKT was evaluated by Western blot after treatment with 10 µmol/L OTP or LPA. A total of 20 µg lysate protein was loaded for each lane. (D) IEC-6 cells were exposed to 25 Gy gamma irradiation 15 minutes after OTP or LPA treatment (10 µmol/L). DNA fragmentation was evaluated 18 hours postirradiation. *P< .05 compared with irradiation alone. **P< .01 compared with irradiation alone. Data shown are means ± SD of at least 3 experiments.