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. Author manuscript; available in PMC: 2012 Sep 19.
Published in final edited form as: Biochim Biophys Acta. 2007 Apr 24;1771(7):883–892. doi: 10.1016/j.bbalip.2007.04.010

Fig. 4.

Fig. 4

Transfection of small interfering RNA (siRNA) of p42 ERK, but not transfection of siRNA of p44 ERK, affects LPA-induced PC3 cell migration. (A) Effect of siRNA of p42 and p44 on expression of these subunits. siRNA of p42, p44 and non-silencing siRNA were transfected into PC3 cells according to the manufacturer’s instructions described in Materials and methods. Specific antibodies against p42 and p44 were used to detect the expression of the ERK subunits. GCK protein served as a loading control. (B) Bar graph of effect of p42 siRNA, p44 siRNA and non-silencing siRNA on p42 protein expression. Results were based on the scanned data of Western blot analyses as illustrated in Fig. 4A. Data were mean±SE from four experiments. (C) Bar graph of effect of p42 siRNA, p44 siRNA and non-silencing siRNA on p44 protein expression. Data were mean±SE of four experiments. D. Effect of p42 siRNA, p44 siRNA and non-silencing siRNA on LPA-induced PC3 cell migration. Data were mean±SE of three experiments. *p<0.05 versus LPA alone.