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. 2012 May 17;6(10):1966–1977. doi: 10.1038/ismej.2012.40

Figure 1.

Figure 1

Phylogeny of marine thaumarchaeal nirK sequences. Maximum likelihood (ML; PhyML, general time-reversible (GTR)+Γ) 30% cutoff consensus tree with 1000 bootstrap replicates, based on 453 bp. Major groupings were all supported by neighbor-joining (NJ, Jukes-Cantor distance correction, 1000 bootstraps). Numbers on branches are bootstrap values from ML/NJ. Only sequence representatives from a 97% similarity OTU cutoff are shown in tree. Colored symbols show total number of clones represented by a sequence (see legend). Sequence name prefix ‘a/b' refers to which primer pair the sequence was amplified with (AnirKa or AnirKb).