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. 2012 Sep 19;3:341. doi: 10.3389/fmicb.2012.00341

Figure 3.

Figure 3

Hybridization of CARD-FISH probes to bacterial epibonts attached to ciliates from Discovery (A,B) and Urania (C,D) haloclines. (A) Epifluorescence of the EUB I-III CARD-FISH probe confirming that the microbes attached to the cortex of a ciliate from Discovery halocline are Bacteria. (B) Epifluorescence of a CARD-FISH probe specific to Delta-proteobacteria, confirming that the microbes attached to the cortex ciliates from Discovery halocline are Delta-proteobacteria. (C,D) Epifluorescence of the EUB I-III bacterial probe hybridized with epibiotic microbes attached to the cortex (as well as internal microbes) of the scuticociliate morphotypes in Urania halocline. Explanations: ma = macronucleus; mi = micronucleus; EB = epibiotic bacteria; B = bacteria (either ingested prey or endosymbionts). Scale bars: (A,B) = 30 μm; (C,D) = 10 μm.