|
Strains
|
|
E. coli EPI300 |
F-
mcrA Δ(mrr-hsdRMS-mcrBC)
Φ80dlacZΔM15 ΔlacX74 recA1 endA1
araD139 Δ(ara, leu)7697 galU galK
λ-
rpsL nupG trfA dhfr; high-transformation efficiency of large DNA |
Epicentre Biotechnologies |
|
E. coli MKH13 |
MC4100Δ(putPA)101D(proP)2D(proU) |
(Haardt et al., 1995) |
|
E. coli MKH13∷pCI372-murB(3) |
MKH13 containing pCI372 with murB gene from SMG 3 (similar to
Collinsella aerofaciens ATCC 25986) |
This study |
|
E. coli MKH13∷pCI372-mazG(3) |
MKH13 containing pCI372 with mazG gene from SMG 3 (similar to
Collinsella aerofaciens ATCC 25986) |
This study |
|
E. coli MKH13∷pCI372-galE(3) |
MKH13 containing pCI372 with galE gene from SMG 3 (similar to
Collinsella aerofaciens ATCC 25986) |
This study |
|
E. coli MKH13∷pCI372-mazG(5) |
MKH13 containing pCI372 with mazG gene from SMG 5 (similar to
Eggerthella sp. YY7918 |
This study |
|
E. coli MKH13∷pCI372-galE(25) |
MKH13 containing pCI372 with galE gene from SMG 25 (similar to
Akkermansia muciniphila ATCC BAA-835) |
This study |
| |
|
|
|
Plasmids
|
|
pCI372 |
Shuttle vector between E. coli and L. lactis,
CmR
|
(Hayes et al., 1990) |
| |
|
|
|
pCC1FOS |
Fosmid cloning vector, CmR
|
Epicentre Biotechnologies |
| |
|
|
|
Transposon
|
|
EZ-Tn5 <oriV/ KAN-2> |
Hyperactive Tn5 transposon, KanR, inducible high copy
origin of replication—oriV |
Epicentre Biotechnologies |
