Figure 5.
Effect of charged lipids on pH-induced release of calcein from PEAA-LUVs.
Notes: Preformed neutral LUVs (40 mol% cholesterol) or charged LUVs (40 mol% cholesterol, and 10 mol% charged lipid introduced) were prepared using an extrusion method with calcein 100 mM. PEAA-LUVs containing calcein were then obtained by post-insertion of PEAA-C10 at a final polymer concentration of 7 mol in PEAA-LUVs containing calcein at a polymer-to-lipid ratio of 20 μg/mg. The calcein released from the PEAA-LUVs was measured after 20 minutes of incubation at room temperature (25°C) as described in the text.
Abbreviations: DODAP, dioleoyl-1,2-diacyl-3-dimethylammonium-propane; DPPC, 1,2 dipalmitoyl-sn-glycero-3-phosphocholine; PI, phosphatidylinositol; DSPE, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine; LUVs, large unilamellar vesicles; PEAA, poly(ethylacrylic acid).