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. Author manuscript; available in PMC: 2012 Sep 19.
Published in final edited form as: Nat Med. 2008 Sep;14(9):979–984. doi: 10.1038/nm.1865

Figure 2.

Figure 2

Pax8-rtTA–mediated, doxycycline-controlled β-galactosidase expression in adult renal tubular cells. (a) β-galactosidase positivity (nuclear staining) in Pax8-rtTA/NZL-2 double-transgenic mice induced with doxycycline was found in all renal tubular epithelial cells, including cortical and medullary regions. (b) Parietal and visceral epithelial cells, as well as mesangial and endothelial cells, of the glomeruli were unstained. Only proximal tubular epithelial cells in a parietal position stained positively in Pax8-rtTA/NZL-2 mice. (c,d) A virtually identical staining pattern to that in b in Pax8-rtTA/LC-1/Rosa26R triple-transgenic mice induced with doxycycline. This experiment uses a transgene encoding tetracycline-inducible Cre recombinase (LC-1) and a gene encoding a cytoplasmic β-galactosidase reporter (Rosa26R). Co, cortex; os, outer stripe of medulla; is, inner stripe of medulla; g, glomerulus; pt, proximal tubular cells in parietal position.