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. Author manuscript; available in PMC: 2012 Sep 19.
Published in final edited form as: Biochemistry. 2010 Mar 9;49(9):1814–1821. doi: 10.1021/bi901924b

FIGURE 4.

FIGURE 4

Incorporation of (R,S)-CEdGTP into template DNA by Kf– under single turnover conditions using primer (8) and template (2). For lanes 1–6, [CEdGTP] in μM: 0, 20, 50, 100, 200, and 500. Calculation of steady-state parameters from these data provided values of KM = 49.7 μM and kcat = 1.36 × 102 M–1 s–1.