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. Author manuscript; available in PMC: 2012 Sep 19.
Published in final edited form as: J Proteome Res. 2011 Mar 2;10(4):1481–1494. doi: 10.1021/pr100877m

Figure 4. In vitro kinase screen identifies CK2 as an eIF3 kinase.

Figure 4

GST-tagged Cka1, Ckb1, and eight control kinases were purified and incubated with [γ-32P]ATP plus purified eIF3 treated with urea to reduce background kinase activity. Reaction products were separated on a 10% SDS-polyacrylamide gel and subjected to autoradiography. In the far right lane, purified eIF3 without urea treatment shows significant endogenous kinase activity. Arrows highlight phosphorylation of eIF3 components above background levels by the Cka1 catalytic subunit and to a lesser extent the Ckb1 regulatory subunit.