Table 1. Mass spectrometry identification of Fun12 and Pab1 interactions with eIF3.
Fun12 protein complexes was isolated from an S. cerevisiae strain expressing TAP-Fun12 and an isogenic, untagged yeast strain as a negative control. Mudpit mass spectrometry analysis of the purified complexes were performed in triplicate. PAF values were used to approximate protein abundances as in Fig. 1.
| Locus | TAP-Fun12 | TAP-Pab1 | negative control |
|
|---|---|---|---|---|
| Targeted Proteins | ||||
| Fun12 | YAL035W | 5.1(1.0) | nda | |
| Pab1 | YER165W | 12(1.0) | nd | |
| eIF3 Core Proteins | ||||
| Rpg1 | YBR079C | 0.18(0.035) | 0.030(0.0025) | nd |
| Nip1 | YMR309C | 0.071(0.014) | 0.036(0.0030) | nd |
| Prt1 | YOR361C | 0.038(0.0074) | nd | nd |
| Tif34 | YMR146C | 0.43(0.084) | nd | nd |
| Tif35 | YDR429C | 0.33(0.064) | nd | nd |
a
nd = none detected