Table 2. Mass spectrometry identification of CK2 interactions with eIF3.
CK2 complexes were isolated from an S. cerevisiae strain expressing TAP-Ckb2 and an isogenic, untagged yeast strain as a negative control. MudPIT mass spectrometry analyses of the purified complexes were performed in triplicate. PAF values were used to approximate protein abundances as in Fig. 1.
| Yeast Protein |
Locus | TAP-Ckb2 | negative control |
|---|---|---|---|
| Cka1 | YIL035C | 6.3(1.2) | nda |
| Cka2 | YOR061C | 5.6(1.0) | nd |
| Ckb2 | YOR029C | 5.5(1.0) | nd |
| Ckb1 | YGL019W | 3.6(0.66) | nd |
| Tif34 | YMR146C | 2.1(0.38) | nd |
| Rpg1 | YBR079C | 2.1(0.39) | nd |
| Tif35 | YDR429C | 1.9(0.34) | nd |
| Nip1 | YMR309C | 1.5(0.27) | nd |
| Prt1 | YOR361C | 1.5(0.28) | nd |
| Tif5 | YBR041W | nd | nd |
a
nd = none detected